Former spy Sergei Skripal and his daughter were poisoned by a military-grade nerve agent of a type developed by Russia, Theresa May has told MPs.
The PM said it was “highly likely” Russia was responsible for the Salisbury attack.
The Foreign Office summoned Russia’s ambassador to provide an explanation.
Mrs May said if there is no “credible response” by the end of Tuesday, the UK would conclude there has been an “unlawful use of force” by Moscow.
The chemical used in the attack, the PM said, has been identified as one of a group of nerve agents known as Novichok.
Mrs May said: “Either this was a direct action by the Russian state against our country, or the Russian government lost control of its potentially catastrophically damaging nerve agent and allowed it to get into the hands of others.”
She said Foreign Secretary Boris Johnson had told the ambassador Moscow must provide “full and complete disclosure” of the Novichok programme to the Organisation for the Prohibition of Chemical Weapons.
Mrs May said the UK must stand ready to take much more extensive measures, and these would be set out in the Commons on Wednesday should there be no adequate explanation from Russia.
Retired military intelligence officer Mr Skripal, 66, and his daughter, Yulia, 33, were found slumped on a bench in Salisbury city centre on Sunday 4 March. They remain in a critical but stable condition in hospital.
Det Sgt Nick Bailey, who fell ill attending the pair, remains seriously ill in hospital but has been talking to his family.
What are Novichok agents?
- The name means “newcomer” in Russian, and applies to a group of advanced nerve agents developed in secret by the Soviet Union in the 1970s and 1980s
- One chemical – called A-230 – is reportedly five to eight times more toxic than VX nerve agent, which can kill a person within minutes.
- Some are liquids, others are thought to exist in solid form. Some are reported to be “binary weapons”, meaning they are typically stored as two less toxic chemicals which when mixed, react to produce the more toxic agent.
- One variant was reportedly approved for use by the Russian military as a chemical weapon.
- Designed to escape detection by international inspectors, what we do know of them comes from scientists who spoke up following the break-up of the USSR.
Addressing the Commons following a meeting of the government’s National Security Council, Mrs May told MPs the positive identification of this chemical agent was made by experts at the UK’s Porton Down laboratory.
She said Russia has previously produced the agent and would still be capable of doing so.
The decision to point the finger at Moscow was also based on “Russia’s record of conducting state-sponsored assassinations and our assessment that Russia views some defectors as legitimate targets for assassinations”, the PM added.
After her speech, the PM’s spokesman said she was yet to speak to senior allies such as USA President Donald Trump, German chancellor Angela Merkel or French president Emmanuel Macron, but there had been “conversations at a senior official level”.
The spokesman also said Mrs May would wait until Moscow responded before involving the UN or Nato but this was not an “article five” matter – a reference to Nato rules which say an attack on one member constitutes an attack on all.
Russian Foreign Ministry spokeswoman Maria Zakharova said Mrs May’s statement was “a circus show in the British parliament”.
“The conclusion is obvious – it’s another information and political campaign based on provocation,” she said.
Earlier, asked whether Russia was to blame, President Vladimir Putin told the BBC: “Get to the bottom of things there, then we’ll discuss this”.
BBC political editor Laura Kuenssberg says the tone of the meeting between Boris Johnson and the Russian ambassador Alexander Yakovenko was “cool but firm”.
She says the men did not shake hands and the foreign secretary expressed the “outrage” of the British public.
Police and Army activity continued on Monday afternoon, with officers – some wearing hazardous materials suits – removing a white van from the village of Winterslow, about six miles from Salisbury.
A Sainsbury’s car park in Salisbury was also sealed off as part of the investigation.
Mr Skripal was convicted by the Russian government of passing secrets to MI6 in 2004, but given refuge in the UK in 2010 as part of a “spy swap”.
On Sunday, up to 500 Salisbury pub-goers and diners were told to wash their possessions as a precaution after trace amounts of the substance used to poison Sergei and Yulia Skripal were found on and around a table where they had eaten in Zizzi.
Traces were also found at the Mill pub in the city which, like Zizzi, remains closed.